{"product_id":"mass-spectrometry-of-glycoproteins-methods-and-protocols-paperback","title":"Mass Spectrometry of Glycoproteins: Methods and Protocols - Paperback","description":"\u003cp\u003eby \u003cb\u003eJennifer J. Kohler\u003c\/b\u003e (Editor), \u003cb\u003eSteven M. Patrie\u003c\/b\u003e (Editor)\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e1. Introduction to Glycosylation and Mass Spectrometry\u003c\/p\u003e\u003cp\u003eSteven M. Patrie, Michael J. Roth and Jennifer J. Kohler \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart I - Enrichment and Isolation Methods\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e2. Tandem Lectin Weak Affinity Chromatography (LWAC) for Glycoprotein Enrichment\u003c\/p\u003e\u003cp\u003eZhi Yuan Ma, Yuliya Skorobogatko, and Keith Vosseller\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e3. CSC Technology - Selective Labeling of Glycoproteins by Mild Oxidation to Phenotype Cells\u003c\/p\u003e\u003cp\u003eAndreas Hofmann, Damaris Bausch-Fluck, and Bernd Wollscheid\u003csup\u003e\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e4. Use of Boronic Acid Nanoparticles in Glycoprotein Enrichment\u003c\/p\u003e\n\u003cp\u003eYawei Xu, Lijuan Zhang and Haojie Lu\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e5. Incorporation of Unnatural Sugars for the Identification of Glycoproteins\u003c\/p\u003e\n\u003cp\u003eBalyn W. Zaro, Howard C. Hang, Matthew R. Pratt\u003c\/p\u003e\n\u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e\n\u003cp\u003e6. Characterization of Membrane-associated Glycoproteins using Lectin Affinity Chromatography and Mass Spectrometry\u003c\/p\u003e\n\u003cp\u003eYashu Liu, Jintang He and David M. Lubman\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e7. Sialic Acid Capture-and-Release and LC-MS\u003csup\u003en\u003c\/sup\u003e Analysis of Glycopeptides\u003c\/p\u003e\n\u003cp\u003eJonas Nilsson and Göran Larson\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003ePart II - Sample Preparation\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e8. In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass Analysis\u003c\/p\u003eEden P. Go, Kathryn R. Rebecchi, and Heather Desaire\u003c\/sup\u003e\u003c\/p\u003e\u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e\u003cp\u003e9. Nano-HPLC-MS of Glycopeptides Obtained After Non-specific Proteolysis\u003c\/p\u003e\u003cp\u003eGerhild Zauner, Carolien A. M. Koeleman, André M. Deelder and Manfred Wuhrer\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart III - Separation Methods\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e10. Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Chromatography Solid Phase Extraction (HILIC SPE)\u003c\/p\u003e\u003cp\u003ePia H nnerup Jensen, Simon Mysling, Peter H jrup and Ole N rregaard Jensen\u003csup\u003e\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e11. Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection\u003c\/p\u003e\n\u003cp\u003eAlina D. Zamfir\u003csup\u003e \u003c\/sup\u003e, Corina Flangea, Alina Serb\u003csup\u003e3\u003c\/sup\u003e, Ana-Maria Zagrean\u003csup\u003e4\u003c\/sup\u003e, Andreas M. Rizzi\u003csup\u003e5\u003c\/sup\u003e and Eugen Sisu\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e12. Structural Separations by Ion Mobility-MS for Glycomics and Glycoproteomics \u003c\/p\u003e\n\u003cp\u003eLarissa S. Fenn and John A. McLean \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003ePart IV - Quantitation Methods\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e13. Quantitative Analysis of Glycoprotein Glycans\u003c\/p\u003e\n\u003cp\u003eRon Orlando\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e14. Stable Isotope Labeling of \u003ci\u003eN\u003c\/i\u003e-glycosylated Peptides by Enzymatic Deglycosylation for Mass Spectrometry-based Glycoproteomics\u003c\/p\u003e\n\u003cp\u003eHiroyuki Kaji and Toshiaki Isobe\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e15. Approaches for Site Mapping and Quantification of O-linked Glycopeptides\u003c\/sup\u003e\u003c\/p\u003e\u003cp\u003ePeng Zhao, Stephanie H. Stalnaker, Lance Wells\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e16. Glycan Profiling: Label-free Analysis of Glycoproteins\u003c\/p\u003e\u003cp\u003eYoshinao Wada\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart V - Computational Tools\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e17. Introduction to Informatics in Glycoprotein Analysis\u003c\/p\u003e\u003cp\u003eKiyoko F. Aoki-Kinoshita\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e18. Software Tools for Glycan Profiling\u003c\/p\u003e\u003cp\u003eChuan-Yih Yu, Anoop Mayampurath, Haixu Tang\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart VI - Case Studies in Mass Spectrometry of Glycoproteins\u003c\/p\u003e\u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e\u003cp\u003e19. Quantitative Characterization of Glycoproteins in Neurodegenerative Disorders using iTRAQ\u003c\/p\u003e\u003cp\u003eMin Shi, Hyejin Hwang and Jing Zhang\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e20. Quantitative Proteomic Analysis of N-linked Glycoproteins in Human Tear Fluid\u003cp\u003eLei Zhou and Roger W. Beuerman\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e21. Elucidation of \u003ci\u003eN\u003c\/i\u003e-glycosites Within Human Plasma Glycoproteins for Cancer Biomarker Discovery\u003c\/p\u003e\u003cp\u003ePenelope Drake, Birgit Schilling, Brad Gibson and Susan Fisher\u003c\/p\u003e\u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e\u003cp\u003e22. Characterizing the Glycosylation State of Therapeutic Recombinant Glycoproteins\u003c\/p\u003e\u003cp\u003eNicole Samuels, David Kates, Jun Liu, Joanne Severs\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e11. Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection\u003c\/p\u003e\u003cp\u003eAlina D. Zamfir\u003csup\u003e \u003c\/sup\u003e, Corina Flangea, Alina Serb\u003csup\u003e3\u003c\/sup\u003e, Ana-Maria Zagrean\u003csup\u003e4\u003c\/sup\u003e, Andreas M. Rizzi\u003c\/p\u003e\u003ch3\u003eBack Jacket\u003c\/h3\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In \u003ci\u003eMass Spectrometry of Glycoproteins: Methods and Protocols, \u003c\/i\u003eexpert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful \u003ci\u003eMethods in Molecular Biology(TM)\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eAuthoritative and practical, \u003ci\u003eMass Spectrometry of Glycoproteins: Methods and Protocol \u003c\/i\u003e is an essential resource for those who work at the interface of glycobiology and mass spectrometry.\u003cdiv\u003e\n\u003cstrong\u003eNumber of Pages:\u003c\/strong\u003e 339\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003eDimensions:\u003c\/strong\u003e 0.73 x 10 x 7 IN\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003eIllustrated:\u003c\/strong\u003e Yes\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003ePublication Date:\u003c\/strong\u003e August 23, 2016\u003c\/div\u003e","brand":"Books by splitShops","offers":[{"title":"Default Title","offer_id":42117402165383,"sku":"9781493962037","price":194.38,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/2623\/2711\/files\/eb9c53729988772cc1286d1bbb73eae3.webp?v=1732524279","url":"https:\/\/booksby.splitshops.com\/products\/mass-spectrometry-of-glycoproteins-methods-and-protocols-paperback","provider":"Books by splitShops","version":"1.0","type":"link"}