{"product_id":"recombinant-protein-production-in-yeast-methods-and-protocols-paperback","title":"Recombinant Protein Production in Yeast: Methods and Protocols - Paperback","description":"\u003cp\u003eby \u003cb\u003eRoslyn M. Bill\u003c\/b\u003e (Editor)\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e1 Optimising Yeast as a Host for Recombinant Protein Production( review)\u003c\/p\u003e\u003cp\u003e Nicklas Bonander\u003csup\u003e \u003c\/sup\u003eand Roslyn M Bill\u003c\/p\u003e\u003cp\u003e2 Which Yeast Species Shall I Choose? \u003ci\u003eSaccharomyces cerevisiae \u003c\/i\u003eversus \u003ci\u003ePichia pastoris\u003c\/i\u003e (review)\u003c\/p\u003e\u003cp\u003e Richard AJ Darby, Stephanie P Cartwright, Marvin V. Dilworth and Roslyn M Bill\u003c\/p\u003e\u003cp\u003e3 Preparation of \u003ci\u003ePichia pastoris\u003c\/i\u003e Expression Plasmids\u003c\/p\u003e\u003cp\u003eChristel Logez, Fatima Alkhalfioui, Bernadette Byrne, Renaud Wagner\u003c\/p\u003e\u003cp\u003e4 Preparation of \u003ci\u003eSaccharomyces cerevisiae\u003c\/i\u003e Expression Plasmids\u003c\/p\u003e\u003cp\u003eDavid Drew and Hyun Kim\u003c\/p\u003e\u003cp\u003e5 Codon Optimisation for Heterologous Gene Expression in Yeast\u003c\/p\u003e\u003cp\u003eKristina Hedfalk\u003c\/p\u003e\u003cp\u003e6 Yeast Transformation to Generate High-yielding Clones\u003c\/p\u003e\u003cp\u003eMohammed Jamshad and Richard AJ Darby\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e7 Screening for High-yielding \u003ci\u003ePichia pastoris\u003c\/i\u003e Clones: the Production of G Protein-Coupled Receptors as a Case Study\u003c\/p\u003e\u003cp\u003eShweta Singh, Adrien Gras, Cedric Fiez-Vandal, Magdalena Martinez, Renaud Wagner, Bernadette Byrne\u003c\/p\u003e\u003cp\u003e8 Screening for High-yielding\u003ci\u003e Saccharomyces cerevisiae \u003c\/i\u003eClones: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins\u003c\/p\u003e\u003cp\u003eDavid Drew and Hyun Kim\u003c\/p\u003e\u003cp\u003e9 The Effect of Antifoam Addition on Protein Production Yields\u003c\/p\u003e\u003cp\u003eSarah J Routledge and Roslyn M Bill\u003c\/p\u003e\u003cp\u003e10 Setting up a Bioreactor for Recombinant Protein Production in Yeast\u003c\/p\u003e\u003cp\u003eSarah J Routledge and Michelle Clare\u003c\/p\u003e\u003cp\u003e11 The Implementation of a Design of Experiments Strategy to Increase Recombinant Protein Yields in Yeast (review)\u003c\/p\u003e\u003cp\u003eNagamani Bora, Zharain Bawa, Roslyn M. Bill and Martin D. B. Wilks\u003c\/p\u003e\u003cp\u003e12 On-line Analysis and Process Control in Recombinant Protein Production (review)\u003c\/p\u003e\u003cp\u003eShane M. Palmer and Edmund R.S. Kunji\u003c\/p\u003e\u003cp\u003e13 Monitoring the Biomass Accumulation of Recombinant Yeast Cultures: Off-line Estimations of Dry Cell Mass and Cell Counts\u003c\/p\u003e\u003cp\u003eShane Palmer and Edmund R S Kunji\u003c\/p\u003e \u003cp\u003e14 On-line Monitoring of Biomass Accumulation in Recombinant Yeast Cultures\u003c\/p\u003e\u003cp\u003eShane Palmer and Edmund R S Kunji\u003c\/p\u003e\u003cp\u003e15 Optimising \u003ci\u003eP. pastoris \u003c\/i\u003eInduction\u003c\/p\u003e\u003cp\u003eZharain Bawa and Richard AJ Darby\u003c\/p\u003e \u003cp\u003e16 Optimizing \u003ci\u003eS. cerevisiae\u003c\/i\u003e Induction Regimes \u003c\/p\u003e\u003cp\u003eDavid Drew and Hyun Kim\u003c\/p\u003e\u003cp\u003e17 Large Scale Production of Membrane Proteins in \u003ci\u003ePichia pastoris: \u003c\/i\u003e The Production of G Protein-coupled Receptors as a Case Study\u003c\/p\u003e\u003cp\u003eShweta Singh, Adrien Gras, Cedric Fiez-Vandal, Magdalena Martinez, Renaud Wagner and Bernadette Byrne\u003c\/p\u003e \u003cp\u003e18 Large-scale Production of Membrane Proteins in \u003ci\u003eSaccharomyces cerevisiae\u003c\/i\u003e: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins\u003c\/p\u003e\u003cp\u003eDavid Drew and Hyun Kim\u003c\/p\u003e\u003cp\u003e19 Large Scale Production of Secreted Proteins in \u003ci\u003ePichia pastoris\u003c\/i\u003e\u003c\/p\u003e\u003cp\u003eNagamani Bora\u003c\/p\u003e\u003cp\u003e20 Disruption of Yeast Cells to Isolate Recombinant Proteins\u003c\/p\u003e\u003cp\u003eMohammed Jamshad and Richard AJ Darby\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003ch3\u003eBack Jacket\u003c\/h3\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eIn the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. \u003ci\u003eRecombinant Protein Production in Yeast: Methods and Protocols\u003c\/i\u003e examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful \u003ci\u003eMethods in Molecular Biology(TM)\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eAuthoritative and practical, \u003ci\u003e Recombinant Protein Production in Yeast: Methods and Protocols, \u003c\/i\u003e seeks to aid scientists in adopting yeast as a protein production host.\u003c\/p\u003e\u003cdiv\u003e\n\u003cstrong\u003eNumber of Pages:\u003c\/strong\u003e 248\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003eDimensions:\u003c\/strong\u003e 0.55 x 10 x 7 IN\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003eIllustrated:\u003c\/strong\u003e Yes\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003ePublication Date:\u003c\/strong\u003e August 23, 2016\u003c\/div\u003e","brand":"Books by splitShops","offers":[{"title":"Default Title","offer_id":42117403869319,"sku":"9781493958719","price":194.38,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/2623\/2711\/files\/f6bd49079c8806569362bc3f42039b1e.webp?v=1732524294","url":"https:\/\/booksby.splitshops.com\/products\/recombinant-protein-production-in-yeast-methods-and-protocols-paperback","provider":"Books by splitShops","version":"1.0","type":"link"}