Trichoderma Reesei: Methods and Protocols - Paperback

by Astrid R. Mach-Aigner (Editor), Roland Martzy (Editor)

1. Ecological Genomics and Evolution of Trichoderma reesei

Komal Chenthamara, Irina S. Druzhinina, Mohammad J. Rahimi, Marica Grujic, and Feng Cai

2. Industrial Relevance of Trichoderma reesei as an Enzyme Producer

Amanda J. Fischer, Suchindra Maiyuran, and Debbie S. Yaver

3. The Potential of Synthetic Biology for Trichoderma reesei

Roland Martzy and Astrid R. Mach-Aigner

4. Resistance Marker- and Gene Gun-Mediated Transformation of Trichoderma reesei

Monika Schmoll and Susanne Zeilinger

5. Use of Auxotrophic Markers for Targeted Gene Insertions in Trichoderma reesei

Irene Tomico-Cuenca and Christian Derntl

6. Open the Pores: Electroporation for the Transformation of Trichoderma reesei

Franziska Wanka

7. Sexual Crossing of Trichoderma reesei

Rita B. Linke

8. CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei

Gen Zou and Zhihua Zhou

9. The Copper-Controlled RNA Interference System in Trichoderma reesei

Lei Wang, Weixin Zhang, Xiangfeng Meng, and Weifeng Liu

Birgit Jovanovic

11. Image Analysis Method for the Characterization of Trichoderma reesei during Fermentations

Nicolas Hardy, Maxime Moreaud, and Fadhel Ben Chaabane

12. Measurement of Cellulase and Xylanase Activities in Trichoderma reesei

Qing-Shan Meng, Fei Zhang, Chen-Guang Liu, Feng-Wu Bai, and Xin-Qing Zhao

13. Flow Cytometry for Filamentous Fungi

Matthias G. Steiger

14. Molecular Identification of Trichoderma reesei

Mohammad Rahimi, Feng Cai, Marica Grujic, Komal Chenthamara, and Irina S. Druzhinina

15. In Vivo Footprinting Analysis in Trichoderma reesei

Alice Rassinger

16. RNA Characterization in Trichoderma reesei

Petra Till

17. Proteomic Profiling of the Secretome of Trichoderma reesei

SoFong Cam Ngan and Siu Kwan Sze

18. Transcriptomics in Trichoderma reesei

Amanda C.C. Antonieto and Roberto N. Silva

19. The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances

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Back Jacket

This detailed book collects standard techniques and cutting-edge methods that are frequently used by the research community studying the fungus Trichoderma reesei. Beginning with overviews of its evolution, its use in the production of industrially-relevant enzymes, and synthetic biology applications, the volume continues with methods covering topics from transformation techniques and gene editing to downstream-analytical applications and -omics analyses and the corresponding bioinformatics approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Trichoderma reesei: Methods and Protocols serves as an ideal guide for a wide range of audiences, from students who want to familiarize themselves with basic research protocols to experienced scientists who are planning to establish a new method in their laboratories, working with this dynamic fungus.